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Ruminant EHDV Serum ELISA Kit

Ruminant EHDV Serum ELISA Kit
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The Kernel EHDV Antibody Assay Kit is designed for the detection of antibodies specific to Epizootic Hemorrhagic Disease (EHD) antigen. EHDV is an orbivirus that infects most wild and domestic ruminants. EHD is endemic in many countries, particularly affecting white-tailed deer in North America, with a morbidity and mortality rate as high as 90%. Deer, elk, and moose can seroconvert. Edema, hemorrhage, and ulcerations are seen in the mouth and lips in the acute form of the disease. In the chronic form of the disease, affected animals exhibit sloughing of the hoof wall, erosions, and scars in the rumen. Spontaneous abortions and stillbirths have also been reported in some epidemics. 

The Kernel EHDV Antibody Test Kit provides a simple, rapid, sensitive and cost-effective enzyme-based immunoassay (ELISA) screening method. 

The method is based on a colorimetric competition ELISA. Purified EHDV antigens have been coated in the plate wells. During analysis, sample (serum or plasma) is added. If antibodies to EHD antigen are present in the sample, it will bind to the coated antigen. A EHDV-specific monoclonal antibody is then added to compete with the available antigen site(s) on the coated plate. A secondary anti-mouse antibody, tagged with a peroxidase enzyme, targets the monoclonal antibody, and the resulting color intensity, after the addition of substrate, is inversely related to the amount of anti-EHDV antibodies in the sample.